Measuring hemoglobin spectra: searching for carbamino-hemoglobin

Heterozygote Hemoglobin J Iran in Combination with Hemoglobin H Disease

This is a report concerning a concurrent case of hemoglobin J Iran (Hb J Iran) and Hemoglobin H (Hb H) disease in an Iranian woman. The patient was coincidentally found during the course of routine pre-marital genetic counselling for her son. The diagnosis of heterozygote Hb J Iran for her son, ultimately led to the diagnosis of concurrent Hb J Iran and Hb H disease. The hematological examinati...

Evaluation of three minicolumn procedures for measuring hemoglobin A1.

We have evaluated three commercially available column-chromatographic methods (Isolab, Helena, and Bio-Rad) for the determination of "fast" hemoglobin (HbA1). All three methods correlated with HbA1c measurements by "high-performance" liquid chromatography for 121 samples from diabetic patients, with the Isolab method showing the highest correlation (r = 0.921). The Isolab and Helena methods gav...

Accuracy of filter paper method for measuring glycated hemoglobin.

BACKGROUND AND OBJECTIVES Glycated hemoglobin (HbA1c) provides an accurate and reliable method to assess the glycemic control in patients with Diabetes. Its measurement is limited by the inconvenience of sample collection that requires venipuncture, sample handling and storage factors. The aim of this study was to assess the feasibility of using a dried capillary blood spot on a filter paper to...

Another step toward standardization of methods for measuring hemoglobin A1c.

Methods for measuring plasma hemoglobin in micromolar concentration compared.

Of eight methods examined for measuring plasma hemoglobin in micromolar concentration, all exhibited acceptable linearity, reproducibility, and concurrence except when specimens were icteric or lipemic or contained methemoglobin or methemalbumin. Measurement of absorbance at 578 nm with an Allen correction permits precise assay of plasma oxyhemoglobin concentration as low as 0.01 g/L (1 mg/dL, ...